Anchor captured read efficiency

We use the following code to generate the plot. This function visualizes the anchor detection efficiency by plotting the percentage of total reads captured and the percentage of anchor-containing reads across different error rates. You can switch the condition argument to analyze other types of error scenarios.

We set four conditions: PCR deletion error rate, Sequencing deletion error rate, Sequencing insertion error rate, and PCR insertion error rate.

uc.vis.anchor_efficiency(
    criteria=criteria,
    quant_captured=pct_reads,
    quant_anchor_captured=pct_anchor_reads,
    condition='PCR deletion error rate',
)

Fig 1. Percentage of captured reads with or without an anchor.

In addition, we can use the following code to visualise it with bars.

uc.vis.anchor_efficiency_simple(
    criteria=criteria,
    quant_captured=pct_reads,
    quant_anchor_captured=pct_anchor_reads,
    condition='PCR deletion error rate',
    # condition='Sequencing deletion error rate',
    # condition='Sequencing insertion error rate',
    # condition='PCR insertion error rate',
)

Fig 1. Percentage of captured reads with or without an anchor.